摘要
采用C18色谱柱(4.6 mm×250 mm,5 μm),以甲醇为流动相A、0.1%磷酸溶液为流动相B,梯度洗脱,(2R,3R)-二氢杨梅素的检测波长为 290 nm,(2S,3S)-二氢杨梅素、花旗松素、杨梅苷和杨梅素的检测波长为 290 nm(0～<21 min)和 255 nm(21～30 min),流速 1 mL/min,柱温 30℃,进样量 10 μL,测定 5种黄酮类物质的含量.结果表明:(2R,3R)-二氢杨梅素、花旗松素、杨梅苷、杨梅素含量的线性回归方程分别为Y=0.503 1X+0.602 8,R2=0.999 2;Y=0.590 4X+0.072 0,R2=0.999 1;Y=0.433 4X-0.007 7,R2=0.999 5;Y=0.507 7X-0.020 7,R2=0.998 8;线性范围分别为10.60～106.00、2.50～50.00、2.65～53.00、1.06～26.50 μg/mL;单波长(290 nm)测定(2R,3R)-二氢杨梅素含量,双波长(290、255 nm)测定花旗松素、杨梅苷、杨梅素含量和(2S,3S)-二氢杨梅素峰面积的方法系统适用性、线性关系、重复性、稳定性和加标回收率良好,精密度高;5种黄酮类物质色谱峰分离度均大于 1.5,(2R,3R)-二氢杨梅素色谱峰理论塔板数大于 5 000,(2S,3S)-二氢杨梅素、花旗松素、杨梅苷、杨梅素色谱峰理论塔板数均大于20 000,且单个样品检测时间为 30 min;此方法可用于检测藤茶中黄酮类物质的含量.
Abstract
C18 column(4.6 mm×250 mm,5 μm)with methanol(A)-0.1%-phosphoric acid solution(B)as the mobile phase for gradient elution was used in the HPLC to measure the contents of five flavonoids.The detection wavelength of(2R,3R)-dihydromyricetin was 290 nm,and the detection wavelength of(2S,3S)-dihydromyricetin,taxifolin,myricitrin and myricetin was 290 nm before 21 min and 255 nm from 21 to 30 min.The flow rate was 1 mL/min,the column temperature was maintained at 30℃,and the injection volume was 10 μL.The results showed that the linear regression equations of the contents of(2R,3R)-dihydromyricetin,taxifolin,myricitrin and myricetin were Y=0.503 1X+0.602 8,R2=0.999 2,Y=0.590 4X+0.072 0,R2=0.999 1,Y=0.433 4X-0.007 7,R2=0.999 5,Y=0.507 7X-0.020 7,R2=0.998 8,and the linear ranges were 10.6-106,2.50-50.00,2.65-53.00,1.06-26.50 μg/mL,respectively.The single-wavelength(290 nm)method for the determination of(2R,3R)-dihydromyricetin content,and the dual-wavelengths(290,255 nm)method for the determination of the contents of taxifolin,myricitrin,myricetin and the peak area of(2S,3S)-dihydromyricetin were superior for system application,linear relationship,repeatability,stability and standard recovery with high precision.The chromatographic peak separations of 5 flavonoids were greater than 1.5,the theoretical plate numbers of(2R,3R)-dihydromyricetin was greater than 5 000,the theoretical plate numbers of(2S,3S)-dihydromyricetin,taxifolin,myricitrin,myricetin were greater than 20 000,and the detection time of single sample was 30 min.This method could be used for the analysis and detection of flavonoids contents in vine tea.
维普
万方数据