Construction of a kiwifruit mutant library and analysis of phenotypic variations
To accelerate the development of functional genomic research,the mutant library of'Hongyang'kiwifruit was constructed.The callus of'Hongyang'kiwifruit were treated with 60Co-γ rays at 10 distinct radiation doses:10,25,50,75,100,150,200,300,400,and 500 Gy,with 0 Gy serving as the control,and the phenotypic variation characteristics of the mutagenic progeny were analyzed.The results indicated that after mutagenic treatment with a dose of 100 Gy,the mutation rate of callus tissue from'Hongyang'kiwifruit increased to 35.9%,while the differentiation rate reached 77.8%.Based on these findings,a radiation dose of 100 Gy was selected as the further studied condition.After treating 1 680'Hongyang'kiwifruit callus under optimal mutagenesis conditions,a kiwifruit mutant library contained 1 026 regenerated seedlings of'Hongyang'kiwifruit was constructed.The mutant library had rich variation in leaf,plant type and other characters,with a total variation frequency of 35.9%.
万方数据
维普
