From January to December 2023,the 1 036 samples were collected from 119 pig farms in 9 representative regions of Hunan Province.The prevalence of Porcine rotavirus(PoRV)were detected by RT-PCR,and then positive VP7 amplification samples was sequenced.Sequence homology was analyzed by MegAlign software.Phylogenetic tree was constructed by using MEGA 7.0 software.Of the 1 036 samples examined,16.22%were positive for PoRV.The prevalence of PoRV in winter was significantly higher than those in other three seasons.Among the 9 sequenced VP7 sequences,G9(77.8%)was the dominant genotype,followed by G4 and G5,both accounting for 11.1%of the total genotypes.These results indicated that the prevalence of PoRV in pig farms was high and the genotypes were complex in Hunan Province.The G9 was the dominant genotype in pig farm in Hunan Province.
维普
万方数据