A method for determining the content of idebenone and coenzyme Q10 in cosmetics by high performance liquid chromatography was established.The samples were ultrasonically extracted with anhydrous ethanol and separated using a Waters XBridge C18 column(250 mm×4.6 mm,5 μm).Gradient elution was performed with anhydrous ethanol-water as the mobile phase at a flow rate of 1.0 mL/min,with column temperature of 40℃and detection wavelength of 275 nm.Quantitative analysis was conducted using the external standard method based on chromatographic peak areas.The mass concentrations of idebenone and coenzyme Q10 had a good linear relationship with the chromatographic peak area in the range of 0.5-100 mg/L,the correlation coefficients were all 0.999 9.The detection limits of idebenone and coenzyme Q10 was 0.11,0.41 mg/kg,respectively,and the quantification limits was 0.37,1.35 mg/kg,respectively.The average recoveries of the four matrices(toner,lotion,cream and gel)at three concentration levels were 94.7%-102.6%,and the relative standard deviations of the determination results was 0.3%-2.8%(n=6).This method is simple and convenient to operate,making it suitable for the determination of idebenone and coenzyme Q10 in cosmetics.
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