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高效液相色谱法同时测定化妆品中25种美白成分

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建立高效液相色谱法同时测定化妆品中25种美白成分.化妆品样品经体积分数为50%的甲醇溶液超声提取,用聚四氟乙烯滤膜过滤,Thermo Scientific AcclaimTM 120(250 mm×4.6 mm,5 μm)色谱柱梯度洗脱分离,二极管阵列检测器检测,外标法定量.25种美白成分的质量浓度在0.4~392.5 μg/mL范围内与色谱峰面积线性关系良好,线性相关系数均大于0.999,在水基、乳剂、粉剂、面膜、凝胶5种基质中的检出限为0.4~41.4 μg/g,定量限为1.3~138.1 μg/g.样品加标平均回收率为92.6%~109.7%,测定结果的相对标准偏差为0.3%~4.8%(n=6).该方法可用于多种化妆品基质中25种美白成分的测定.
Simultaneous determination of 25 whitening ingredients in cosmetics by high performance liquid chromatography
A method for simultaneous determination of 25 whitening components in cosmetics by high performance liquid chromatography was established.Cosmetic samples were extracted by 50%(volume fraction)methanol solution ultrasound,filtered by polytetrafluoroethylene(PTFE)membrane,separated by gradient elution on Thermo Scientific AcclaimTM 120(250 mm×4.6 mm,5 μm)chromatography column,detected by diode array detector(DAD),and quantified by external standard method.The mass concentration of 25 whitening components had a good linear relationship with the chromatographic peak area in the range of 0.4-392.5 μg/mL,and the linear correlation coefficients were all more than 0.999.The detection limits were 0.4-41.4 μg/g and the quantification limits were 1.3-138.1 μg/g in water base,emulsion,powder,facial mask and gel.The average recoveries of spiked samples were 92.6%-109.7%,and the relative standard deviations of determination results were 0.3%-4.8%(n=6).This method can be used for the determination of 25 whitening components in most cosmetic matrices.

cosmeticfreckle-removingwhiteninghigh performance liquid chromatography

吴姣娇、赵薇、潘小红、涂晓琴、谭雪莹、殷帅、陈真

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湖南省药品检验检测研究院,湖南省药品质量评价工程技术研究中心,长沙 410000

湖南省药品审核查验中心,长沙 410000

化妆品 祛斑 美白 高效液相色谱法

2025

化学分析计量
中国兵器工业集团第五三研究所(国防科技工业应用化学一级计量站)

化学分析计量

影响因子:0.444
ISSN:1008-6145
年,卷(期):2025.34(1)