A high performance liquid chromatography internal standard method for determining the content of F-Ketamine(2F-DCK)in drugs was established.The sample was weighed,dissolved,shaken evenly,and filtered,then analyzed on a Waters symmetry®C18(250 mm×4.6 mm,5 μm)chromatographic column,with phosphate acid-triethylamine solution and methanol as the mobile phase for isocratic elutionthe(the volume was 70∶30),and the detection wavelength was 210 nm.The mass concentration of F-Ketamine had a good linear relationship with chromatographic peak area in the range of 2-500 mg/L,with a correlation coefficient of 0.999 9.The detection limit of the method was 1 mg/L,and the quantification limit was 2 mg/L.The recoveries of low,medium,and high levels of spiked samples were 99.0%-102.2%,and the relative standard deviations of the measurement results were 0.71%-4.05%(n=6).The method has good separation effect and stability,which is suitable for quantitative analysis of F-Ketamine in solid drugs.
关键词
氟胺酮/高效液相色谱法/内标法/毒品
Key words
F-Ketamine/high performance liquid chromatography/internal standard method/drugs
维普
万方数据