A method for the determination of cosmetics raw materials copper tripeptide-1(GHK-Cu)by high performance liquid chromatography was established,and to investigate its high temperature and light stability.After dissolving in 0.1%trifluoroacetic acid solution,the samples were separated by CAPCELL PAK ADME C18 column.0.1%trifluoroacetic acid solution was used as the mobile phase with isocratic elution,the flow rate was 1.0 mL/min,and the column temperature was 30℃.The samples were determined by DAD detector,the detection wavelength was 217 nm,and the sample volume was 10 μL.Quantify by chromatographic peak area external standard method.The mass concentration of tripeptide-1 had a good linear relationship with the chromatographic peak area in the range of 0.937 5-93.75 μg/mL,and the correlation coefficient was 0.999 9.The detection limit of GHK-Cu was 1.1 ng,and the quantification limit was 3.3 ng.The recoveries of samples spiked were 100.2%-102.1%,and the relative standard deviation of the measurement results was 0.58%(n=9).This method can meet the requirements of rapid and accurate determination of copper tripeptide-1.
关键词
三肽-1铜/高效液相色谱法/化妆品原料
Key words
copper tripeptide-1/high performance liquid chromatography/raw materials of cosmetics
维普
万方数据