Laccase-like activity of Mn3O4 nanoparticles and its application in degrading acridine orange
Natural laccase suffers from several inherent drawbacks,such as complex purification process,poor stability and high cost,significantly constraining their practical applications.The construction of in-organic nanozymes with laccase activity is a promising strategy to overcome these limitations.In this study,Mn3O4 nanoparticles were prepared using a chemical precipitation method.The laccase-like activity was evaluated using 2,4-dichlorophenol as the substrate.Additionally,the removal performance of the nanop-articles towards AO in aqueous solution was investigated.This study shows that Mn3O4 nanoparticles have redox activity similar to laccase,and enzyme kinetics experiments demonstrate that Mn3O4 nanozyme has higher catalytic efficiency than natural laccase at the same concentration.Furthermore,Mn3O4 nanozyme have a wider temperature(25-75 ℃)and pH(6-7)tolerance range,and the storage stability at room temperature is significantly higher than that of natural laccase.In the presence of the redox mediator HBT,Mn3O4 nanoparticles can swiftly and effectively remove AO from aqueous solution,with a removal efficiency of 70％in just 5 min.Therefore,Mn3O4,as a laccases-like nanozyme,has excellent develo-ping potential in treatment of dye wastewater due to high catalytic activity,superior storage stability,sim-plicity of preparation and low cost.
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