Correlation between FOXN3-SIN3A complex expression in peripheral blood and non-syndromic cleft lip and palate in Xinjiang
Objective This work aimed to study the correlation between FOXN3-SIN3A complex expression and non-syndromic oral clefts(NSOC)in Xinjiang.Methods In this study,60 patients with NSOC attending the People's Hospital of Xinjiang Uygur Autonomous Region were recruited into the case group,including 30 cleft lip with or without cleft palate(NSCL/P),30 cleft palate only(CPO),and 30 healthy children in the control group.The expression levels of FOXN3,SIN3A,and NEAT1 in peripheral blood of each group were detected by high-throughput second-generation se-quencing technology and quantitative reverse transcription polymerase chain reaction(RT-qPCR).Receiver operating characteristic(ROC)curve and area under the curve(AUC)were used to analyze the diagnostic efficiency of NSOC.Re-sults The comparison of the NSOC and control groups showed that FOXN3,SIN3A,and NEAT1 genes increased com-pared with the control group.The differences were all sta-tistically significant(P<0.05).The AUCs of FOXN3,SIN3A,and NEAT1 in the NSCL/P group were 0.933[95%CI=(0.864,1.000)],0.822[(95%CI=(0.713,0.932)],and 1.000[95%CI=(1.000,1.000)],respectively.The AUCs of FOX-N3,SIN3A,and NEAT1 in the CPO group were 0.891[95%CI=(0.806,0.976)],0.688[95%CI=(0.552,0.824)],and 1.000[95%CI=(1.000,1.000)],respectively.Conclusion The results showed a correlation between the ris-ing gene expression of FOXN3,SIN3A,and NEAT1 in peripheral blood and the occurrence of NSOC in Xinjiang.This work provides a theoretical basis for further study of the FOXN3-SIN3A complex as biomarkers to facilitate the early screening,disease prediction,and early prevention of NSOC.
non-syndromic cleft lip and palateFOXN3SIN3ANEAT1biomarker
国家科技期刊平台
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