首页|基于紫外拉曼光谱的阳离子与酰胺相互作用研究

基于紫外拉曼光谱的阳离子与酰胺相互作用研究

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蛋白质与盐离子的相互作用在生命活动中起着重要作用.本文使用丁酰胺作为模型分子,通过紫外拉曼光谱观察丁酰胺与金属氯化物水溶液的结合,研究蛋白质骨架与阳离子的相互作用.发现了水合良好的金属阳离子(Ca2+,Mg2+)倾向于结合酰胺键中与C=O,从而使得酰胺Ⅰ谱带进行重新分配.具体而言,1655 cm-1处与1610 cm-1处的峰强度比值随浓度的增加而显著增加,此现象在NaCl溶液中并未明显观察到.本文还研究了盐离子对水结构的影响,Ca2+和Mg2+的加入有利于水在3400 cm-1位置的信号增强,而相同浓度下的Na+则不明显.研究表明,阳离子与酰胺的相互作用满足以下顺序:Ca2+＞Mg2+＞Na+,符合霍夫迈斯特序列.
Investigation on Binding between Cations and Amides using UV Raman Spectroscopy
The interaction of proteins with salt ions plays an impor-tant role in life activities.We used butyramide as a model molecule to investigate the interaction of protein back-bones with cations.The experiment was performed in an aqueous solution of metal chloride using UV Raman spec-troscopy.It was found that well-hydrated metal cations(Ca2+,Mg2+)tend to bind to C=O in the amide bond,re-sulting in redistribution of the amide I band peaks.Specifi-cally,the peak intensity ratio of 1655 cm-1 to 1610 cm-1 in-creases significantly with increasing concentrations.However,this phenomenon is not obvi-ously observed in NaCl solution.Furthermore,we studied the effect of salt ions on the water structures.The addition of Ca2+and Mg2+is beneficial to the enhancement of the water sig-nal at the 3400 cm-1 position,while the Na+at the same concentration is not obvious.The re-sults have shown that the interaction between cations and amides satisfies the following or-der:Ca2+＞Mg2+＞Na+,which conforms to the Hofmeister series.

UV Raman spectroscopyButyramideHofmeister

刘艺璇、王周兵、王静静、田康振、孟鑫、毛桂林

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江苏师范大学物理与电子工程学院,徐州 221116

江苏省先进激光材料与器件重点实验室,徐州 221116

紫外拉曼光谱 丁酰胺 霍夫迈斯特

National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNatural Science Foundation of the Higher Education Institutions of Jiangsu ProvinceGraduate Research and Practice Innovation Program of Jiangsu Normal UniversityGraduate Research and Practice Innovation Program of Jiangsu Normal University

620051086220513421KJB1400082021XKT12012021XKT1204

2024

10.1063/1674-0068/cjcp2301008

化学物理学报(英文版)
中国物理学会

化学物理学报(英文版)

CSTPCDEI
影响因子：0.162
ISSN：1674-0068
年,卷(期)：2024.37(4)