Quantitative changes analysis between contents of active ingredients and trace elements in Scutellaria Barbata D. Don
Determination of the contents of trace elements and active ingredients in the medicinal plant Scutellaria barbata which grew the different areas,to discuss the relationships between its various constituents,so as to provide a reference for the quality eval-uation of S. barbata. The contents of 9 trace elements in S. barbata were detected using flame atomic absorption spectrometry. HPLC was used to determine the content of scutellarin. Total flavonoids and polysaccharides were determined by UV-Vis method. The cor-relations between contents of active ingredients and trace elements in S. barbata were analyzed by correlation analysis and regression analysis. Cluster analysis method was employed to evaluate the quality of S. Barbata from different areas. The result of correlation a-nalysis showed that scutellarin and total flavonoids of S. barbata had a strong positive correlation with Zn content and had significant positive correlation with Mn content. Polysaccharides had strong positive correlation with Zn and Mn contents. And on this basis,the corresponding regression equation models were established. Through using the clustering figure,the similarities and differences a-mong the quality of S. barbata from different habitats were explained. The contents of active ingredients of S. barbata closely related with the trace elements,but the concrete quantitative relationship still need further study. Therefore,the research can provide some reference for cultivation and the quality evaluation of S. barbata.