Colorimetric detection of uric acid based on AgPt nanoparticles with peroxidase mimics
AgPt alloy nanoparticles were synthesized in a two-step method,and their morphology and composition were characterized by transmission electron microscopy,X-ray powder diffraction,and X-ray photoelectron spectroscopy.The results show that the pre-pared materials are AgPt alloy nanoparticles with a particle size of approximately 50 nm.The resultant nanoparticles have good per-oxidase mimic activity,and in the presence of hydrogen peroxide,can catalyze the oxidation of the colorless peroxidase substrate 3,3',5,5'-tetramethylbenzenzidine(TMB)to produce the blue oxidation product(ox-TMB).The synthesis conditions and the simula-ted enzyme reaction conditions were optimized,and the mechanism of peroxidase mimic activity were also investigated.Finally,col-orimetric detection of uric acid was constructed based on the cascade between uricase and AgPt nanozyme with TMB as the sub-strate.The linear range of uric acid detection was 5-150 μM,with a detection limit of 2.4 μM.
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