Synthesis of cholesterol-quinoline conjugates and their recognition properties for Zn2+and trivalent ions
A molecular probe C for the conjugation of quinoline at the three positions of cholesterol was designed and synthesized.The structure was characterized by1HNMR、13C NMR and HREI-MS,and its recognition performance for metal ions was studied.The fluorescence spectrum experiment found that the fluorescence intensity of the molecular probe C was weak in ethanol solution.After adding Zn2+,its fluorescence intensity increased by 6.1 times at 445 nm,while Cd2+only increased by 2.8 times.Cr3+,Fe3+,and Al3+could cause the fluorescence peak of the molecular probe C to shift red by 52.4 nm,52.2 nm,and 53.4 nm,respectively,and emit blue-green fluorescence under UV lamp.Through titration experiments,it was known that there was a good linear relation-ship between molecular probe C and metal ions within a certain concentration range,and its complexation constant was calculated.Taking Zn2+as an example,further studied the complexation mechanism between probe C and Zn2+through Job curves,reference mo-lecular recognition performance,and nuclear magnetic titration experiments.Probe C was found to be 1∶1 complexed with Zn2+,and coordinated with Zn2+through quinoline nitrogen atoms,oxygen atoms,and carbonyl groups in ester groups.This study enriched the research on the recognition of heavy metal ions by steroid molecules.
万方数据
