Synthesis and application of a hemicyanine-based reactive fluorescent probe for the detection of Cu2+
A reaction-based fluorescent probe BITPP was designed and synthesized by using hemicyanine dye as the fluorophore and the 2-picolinic ester group as the recognition unit,and the structure was characterized by NMR,IR,and HRMS.The solvent medium for the detection of Cu2+by probe BITPP was optimized,and the mixed solvent of EtOH/HEPES(V/V,1/1,pH=7.0)was estab-lished as the optimal test condition for this study.Under this condition,a detailed study was conducted on the properties of the probe BITPP for the detection of Cu2+.The results showed that the probe was highly selective to Cu2+without interference from other coex-isting metal ions.The fluorescence intensity of the BITPP probe displayed a strong linear correlation with the Cu2+concentration,spanning the range of 0.099 5 to 2.5 μmol·L-1(R2=0.991 5),with a detection limit of 99.5 nmol·L-1.The mechanism study confirmed that Cu2+promoted the hydrolysis of the 2-picolinic ester group,resulting in charge transfer fluorescence enhancement,thereby realizing the qualitative and quantitative detection of Cu2+.The fluorescent probe BITPP had been shown to be useful for the detection of Cu2+in real water samples by means of a spiked recovery experiment.
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