Establishment and optimization of PCR-DGGE technique on endophytic fungi for Ligusticum chuanxiong
OBJECTIVE To optimize the ploymerase chain reaction and denature gradient gel electrophoresis (PCR-DGGE)technique that was used to analyze the community composition of endophytic fungi for Ligusticum chuanxiong Hort.METHODS The gradient range and electrophoresis time of PCR-DGGE system based on its region were optimized by using rhizomatic material of cultivated Ligusticum chuanxiong.RESULTS The results suggest that the best denaturant gradient range is from 45％ to 80％,the optimal electrophoresis time is 160 min.CONCLUSION This method is simple and quick,and layed a foundation for the further study on the analysis of the community of endophytic fungi for Ligusticum chuanxiong.
NETL
NSTL
万方数据
维普
