Network pharmacological study on key targets of Chaihu Zaoxiu decoction in the treatment of hepatocellular cancer and establishment of prognostic models
OBJECTIVE To investigate the key targets of Chaihu Zaoxiu decoction(CZD)in the treatment of hepatocellular carcinoma using network pharmacology,and to construct a risk score model to address the prognosis of hepatocellular carcinoma by quantitative means.METHODS The main medicinal active ingredients,the molecular targets of action and the prognostic ability of related genes in CZD for the treatment of hepatocellular carcinoma were investigated by network pharmacology,LASSO COX modelling and in vitro cellular assay methods.RESULTS CZD may act through key targets of ESR1,G6PD,AKR1C3,NQ01 and CDK1,and the p53 signaling pathways to treat hepatocellular carcinoma.Molecular docking results showed good binding of these key targets to the active ingredients.The risk score model based on LASSO COX indicated that it owned good prognostic effect and independent prognostic ability.In vitro experiments demonstrated that CZD was concentration-dependent and time-dependent in its cytotoxicity against human hepatoma HepG2 cells and was able to significantly inhibit the expression of two core targets,G6PD and CDK1.CONCLUSION CZD can treat hepatocellular carcinoma by modulating key targets of G6PD and CDK1,and signaling pathway of p53,and the novel risk scoring model built with G6PD and CDK1 indicates good independent prognostic function for hepatocellular carcinoma.This study provides new ideas for subsequent network pharmacology research.
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