三七总皂苷对调节性T细胞再分化为辅助性T细胞的影响
Effects of total saponins of Panax notoginseng on re-differentiation of Treg into Th17 cells
田锋祥 1张明菲 1申美玉 1狄昱希 1周玲玲1
作者信息
- 1. 南京中医药大学药学院,江苏南京 210023
- 折叠
摘要
目的 考察三七总皂苷(PNS)调控调节性T细胞(Treg)再分化为辅助性T细胞17(Th17)的作用.方法 分离C57/BL6小鼠脾细胞,并磁珠分选出CD4+T细胞,进行体外培养,采用CCK-8法筛选PNS合适的给药浓度.建立Treg再分化为Th17的细胞模型,采用流式细胞术检测PNS对Treg再分化体系中Th17细胞分化比例的影响,酶联免疫吸附法检测体系上清中Th17特征性细胞因子白细胞介素(IL)-17A的水平,实时荧光定量聚合酶链式反应检测Th17特征性转录因子RORγt mRNA及炎症因子IL-17 mRNA的表达.结果 PNS可显著降低Treg再分化体系中CD4+IL-17+Th17细胞的比例和IL-17A的分泌水平,抑制ROR-γt mRNA和IL-17 mRNA的表达.结论 一定浓度的PNS可抑制Treg再分化为Th17,调控Treg/Th17平衡,进而防治类风湿关节炎等炎症免疫性疾病的发生和发展.
Abstract
OBJECTIVE To investigate the effect of total saponins of Panax notoginseng(PNS)on the trans-differentiation of Treg into Th17.METHODS The spleen cells of C57/BL6 mice were isolated and CD4+T cells were cultured in vitro by magnetic beads sorting.The appropriate concentration of PNS was screened by CCK-8 method,based on the index of cell viability.The cell model of Treg trans-differentiation into Th17 was established.The effect of PNS on the differentiation ratio of Treg/Th17 cells was detected by using flow cytometry,the level of Th17 cell characteristic cytokine IL-17A was measured by ELISA,and the Th17 cell characteristic transcription factor RORγt mRNA and inflammatory factor IL-17 mRNA was detected by real-time fluorescence quantitative polymerase chain reaction.RESULTS PNS could significantly inhibit the proportion of CD4+IL-17+Th17cells,the secretion of cytokine IL-17A and RORγt mRNA expression.CONCLUSION Certain concentrations of PNS can inhibit the trans-differentiation of Treg into Th17 and regulate the proportion balance of Treg/Th17 cells,and then prevent the occurrence and development of inflammatory immune diseases including rheumatoid arthritis.
关键词
类风湿关节炎/滑膜炎症/三七总皂苷/调节性T细胞/辅助性T细胞17/再分化/RORγt/白细胞介素-17AKey words
Rheumatoid arthritis/Synovial inflammation/Total saponins of Panax notoginseng/Treg/Th17/Trans-differentiation/RORγt/IL-17A引用本文复制引用
基金项目
国家自然科学基金资助项目(82174306)
出版年
2024
NETL
NSTL
万方数据