Study on the effects and mechanism of botanical compound MYD-20 against Staphylococcus aureus
OBJECTIVE To investigate the inhibitory effect and preliminary mechanism of the botanical compound MYD-20 and its ointment preparation on Staphylococcus aureus.METHODS The minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of the clinical strains of Staphylococcus aureus and Methicillin-resistant S.aureus(MRSA)were determined by broth dilution method.The mechanism of antibacterial effect of MYD-20 was determined by the alkaline phosphatase activity and extracellular macromolecule concentration of the drug-treated bacterial solution as well as scanning electron microscopy.RESULTS MYD-20 showed strong antibacterial activity against S.aureus and MRSA,with MIC and MBC of 1.0％and 2.0％against both S.aureus and MRSA,respectively.AKP activity and extracellular macromolecule content in the bacterial solution were significantly increased after MYD-20 treatment,and combined with SEM observation,it could be found that the surface MYD-20 ointment showed significant wound healing effect and reduced the levels of inflammatory factors at the wounds in the model mice with deep Ⅱ degree skin scald infection.CONCLUSION The compound MYD-20 has a strong antibacterial effect on S.aureus and MRSA,and its mechanism is related to disrupting the permeability and integrity of bacterial cell membranes and cell walls,leading to leakage of cell contents.In addition,MYD-20 ointment can effectively reduce the level of inflammation at the wounds of scald-infected mice,as well as promote the healing effect of the wounds.
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