植物药复方MYD-20抗金黄色葡萄球菌的作用及机制研究
Study on the effects and mechanism of botanical compound MYD-20 against Staphylococcus aureus
戴晓婷 1陈思 1周代红 1茆灿泉1
作者信息
- 1. 西南交通大学生命科学与工程学院,四川成都 610031
- 折叠
摘要
目的 研究植物药复方MYD-20及其软膏剂对金黄色葡萄球菌的抑制作用及初步作用机制.方法 采用肉汤稀释法测定复方MYD-20对金黄色葡萄球菌Staphylococcus aureus、耐甲氧西林金黄色葡萄球菌Methicillin-resistant S.aureus(MRSA)临床菌株的最低抑菌浓度(MIC)、最低杀菌浓度(MBC);通过测定药物处理后菌液的碱性磷酸酶(AKP)活性、胞外大分子浓度变化以及扫描电子显微镜的观察结果确定复方MYD-20的抑菌作用机制;建立小鼠烫伤感染模型,探究MYD-20软膏剂的药效作用.结果 MYD-20对金黄色葡萄球菌和MRSA表现出强抑菌活性,对金黄色葡萄球菌、MRSA的MIC和MBC均分别为1.0％、2.0％.经MYD-20处理后,菌液中AKP的活性、胞外大分子的含量均显著上升,结合扫描电子显微镜的观察结果,发现细菌的表面结构受到破坏.MYD-20软膏剂对皮肤深Ⅱ度烫伤感染的模型小鼠显示出显著的创口愈合作用;并降低了创口处炎症因子的表达.结论 复方MYD-20对金黄色葡萄球菌和MRSA具有较强的抗菌作用,其作用机制与破坏细菌细胞膜、细胞壁的通透性和完整性,导致细胞内容物泄露有关.此外,MYD-20软膏剂能有效降低烫伤感染小鼠创口处的炎症水平,促进创口愈合.
Abstract
OBJECTIVE To investigate the inhibitory effect and preliminary mechanism of the botanical compound MYD-20 and its ointment preparation on Staphylococcus aureus.METHODS The minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of the clinical strains of Staphylococcus aureus and Methicillin-resistant S.aureus(MRSA)were determined by broth dilution method.The mechanism of antibacterial effect of MYD-20 was determined by the alkaline phosphatase activity and extracellular macromolecule concentration of the drug-treated bacterial solution as well as scanning electron microscopy.RESULTS MYD-20 showed strong antibacterial activity against S.aureus and MRSA,with MIC and MBC of 1.0％and 2.0％against both S.aureus and MRSA,respectively.AKP activity and extracellular macromolecule content in the bacterial solution were significantly increased after MYD-20 treatment,and combined with SEM observation,it could be found that the surface MYD-20 ointment showed significant wound healing effect and reduced the levels of inflammatory factors at the wounds in the model mice with deep Ⅱ degree skin scald infection.CONCLUSION The compound MYD-20 has a strong antibacterial effect on S.aureus and MRSA,and its mechanism is related to disrupting the permeability and integrity of bacterial cell membranes and cell walls,leading to leakage of cell contents.In addition,MYD-20 ointment can effectively reduce the level of inflammation at the wounds of scald-infected mice,as well as promote the healing effect of the wounds.
关键词
植物药复方/金黄色葡萄球菌/耐甲氧西林金黄色葡萄球/抑菌活性/梯度稀释法/软膏剂/最低抑菌浓度/最低杀菌浓度/深Ⅱ度烫伤感染Key words
Botanical compound/Staphylococcus aureus/MRSA/Antibacterial activity/Gradient dilution method/Ointment/MIC/MBC/Deep Ⅱ degree burn infection引用本文复制引用
基金项目
四川省科技厅科技计划重点项目(2022YFS0437)
出版年
2024
NETL
NSTL
万方数据