扫日劳清肺止咳胶囊中没食子酸的测定及超声提取工艺的优化
Detection and optimization of ultrasonic extraction technology of gallic acid in Saorilao Qingfeizhike capsule
邹继红1
作者信息
- 1. 赤峰学院基础医学院,内蒙古赤峰 024000
- 折叠
摘要
目的 测定扫日劳清肺止咳胶囊中的没食子酸并优化其超声提取工艺.方法 采用Hypersil BDS C18色谱柱,以甲醇-乙腈-0.1％磷酸(5∶5∶90)为流动相,在260 nm处测定扫日劳清肺止咳胶囊中的没食子酸.以没食子酸的含量为评价指标、甲醇溶液为溶剂,通过单因素试验和正交试验测定甲醇浓度、超声温度、固液比、超声时间等因素的影响,确定最优提取条件.结果 最佳提取工艺为:甲醇浓度70％、超声温度50 ℃、固液比1∶80、超声时间10 min;4.04～40.4 μg·mL-1没食子酸与峰面积具良好的线性关系(r=0.9999),扫日劳清肺止咳胶囊中没食子酸的平均含量为4.83 mg·g-1.结论 优化后的超声提取条件简便可行,没食子酸的提取含量高,测定结果准确.
Abstract
OBJECTIVE To detect and optimize the ultrasonic extraction technology of gallic acid in Saorilao Qingfeizhike capsule(SQC).METHODS The separation was conducted on a Hypersil BDS C18 column and the detection wavelength was 260 nm.The mobile phase consisted of methanol,acetonitrile and 0.1％phosphoric acid(5∶5∶90).Based on the gallic acid content,using methanol solution as the solvent,the four influencing factors of methanol concentration,ultrasonic temperature,material-liquid ratio and ultrasonic time were determined by single factor test and orthogonal test to determine the optimal extraction conditions.RESULTS The optimum extraction process was as follows:methanol concentration of 70％,ultrasonic temperature at 50 ℃,material-liquid ratio of 1∶80,ultrasonic time of 10 min.Gallic acid had a good linear relationship with the peak area in the range of 4.04-40.4 µg·mL-1(r=0.9999).The average content of gallic acid in SQC was 4.83 mg·g-1 by HPLC.CONCLUSION The optimized ultrasonic extraction conditions have proven to be simple and feasible with high gallic acid extraction and accuracy.
关键词
扫日劳清肺止咳胶囊/高效液相色谱法/没食子酸/正交试验/超声提取/优化/蒙药/提取工艺Key words
Saorilao Qingfeizhike capsule/HPLC/Gallic acid/Orthogonal test/Ultrasonic extraction/Optimization/Mongolian medicine/Extraction process引用本文复制引用
基金项目
内蒙古人类遗传病研究自治区高等学校重点实验室开放课题资助项目(YC202301)
出版年
2024
NETL
NSTL
万方数据