Determination of three chemical components in pomegranate seeds by HPLC
OBJECTIVE To study the changes of fructose,glucose,and 5-hydroxymethylfurfural(5-HMF)contents in pomegranate seeds under different drying temperatures and drying times,and to provide reference for quality evaluation of pomegranate seeds.METHODS Pomegranate medicinal materials were collected and placed in an oven at set temperatures of 60,80,and 105 ℃.The samples were collected after 1,4,8,12,18,24,36,and 48 h,respectively.The Mercury NH2 column(250 mm×4.6 mm,5 μm)was used with acetonitrile-water(70∶30)as the mobile phase for elution.The flow rate was 0.5 mL·min-1 and the column temperature was 25 ℃.The content of fructose and glucose in the sample was determined by differential refractive index detector.Agilent Zorbax SB-C18 column(250 mm ×4.6 mm,5 μm)was used with the mobile phase of acetonitrile-0.1％formic acid(2∶98)at a flow rate of 1.0 mL·min-1.The column temperature was 30 ℃ and the detection wavelength was 284 nm.The content of 5-HMF in samples was determined.The correlation between drying temperature,drying time and fructose,glucose,5-HMF content was analyzed by SPSS 26.0 software.RESULTS With the increase of drying temperature and drying time,the contents of fructose and glucose in pomegranate seeds decreased,and the content of 5-hydroxymethylfurfural increased.When the temperature was set at 80 ℃ and 105 ℃,the change was more obvious.Drying temperature and drying time were significantly correlated with glucose content,fructose content and 5-HMF content.Glucose content and fructose content were negatively correlated,and 5-HMF content was positively correlated.CONCLUSION During the drying process of pomegranate seeds,the temperature should be kept below 60 ℃,and the time should not exceed 48 h.The method established in this study has been proven to be accurate and reproducible,which can provide reference for the formulation of quality standards and production quality control of pomegranate seeds.
HPLCSeed of pomegranateDrying temperatureDrying timeFructoseGlucose5-hydroxymethylfurfuralContent determinationQuality control
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