Osteogenic differentiation induction effect of Periplaneta americana extracts on rat bone marrow mesenchymal stem cells
OBJECTIVE To determine the osteogenic differentiation-inducing effect of four Periplaneta americana extracts(PAE)on rat bone marrow mesenchymal stem cells(bMSCs).METHODS bMSCs were cultured with each of the four extracts.After 14 days of induction,the gene transcription levels of type Ⅰ collagen α1 and alkaline phosphatase were determined by fluorescence quantitative PCR,the protein activity of alkaline phosphatase was determined by colorimetry,and the protein levels of osteocalcin and osteopontin were determined by immunocytochemistry.After 21 days of induction,the formation of calcified nodules was observed by alizarin red staining.RESULTS The indices of osteogenic differentiation detected in each treatment group increased with different extent compared with the control group.Some increases were significant.XD02 had the best inductive effect and the least cytotoxicity.CONCLUSION The results indicates that all four PAE could induce osteogenic differentiation of bMSC.The PAE has the potential to be developed as a dietary supplement or drug for osteoporosis intervention.
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