美洲大蠊提取物对大鼠骨髓间充质干细胞的成骨分化诱导作用
Osteogenic differentiation induction effect of Periplaneta americana extracts on rat bone marrow mesenchymal stem cells
马秀英 1葛燕 1刘彬 1吴桃清 1姜顺日 1刘姝 1沈咏梅 1耿福能1
作者信息
- 1. 四川好医生攀西药业有限责任公司药用美洲大蠊四川省重点实验室,四川西昌 610000
- 折叠
摘要
目的 测定4种美洲大蠊提取物对大鼠骨髓间充质干细胞(bMSCs)的成骨分化诱导作用.方法 用4种提取物分别对bMSCs进行诱导培养.14 d后,用qPCR测定Ⅰ型胶原α1和碱性磷酸酶的基因转录水平,比色法测定碱性磷酸酶的活性,免疫细胞化学法检测骨钙素、骨桥蛋白的水平.21 d后,用茜素红染色观察钙化结节的形成情况.结果 与对照组比较,4种美洲大蠊提取物各处理组检测出的成骨分化指标均有不同程度升高,某些浓度下差异显著.其中,XD02的诱导效果最好,且细胞毒性最小.结论 4种美洲大蠊提取物均具诱导骨髓间充质干细胞成骨分化的作用.美洲大蠊提取物有望成为干预骨质疏松的营养添加剂或药物.
Abstract
OBJECTIVE To determine the osteogenic differentiation-inducing effect of four Periplaneta americana extracts(PAE)on rat bone marrow mesenchymal stem cells(bMSCs).METHODS bMSCs were cultured with each of the four extracts.After 14 days of induction,the gene transcription levels of type Ⅰ collagen α1 and alkaline phosphatase were determined by fluorescence quantitative PCR,the protein activity of alkaline phosphatase was determined by colorimetry,and the protein levels of osteocalcin and osteopontin were determined by immunocytochemistry.After 21 days of induction,the formation of calcified nodules was observed by alizarin red staining.RESULTS The indices of osteogenic differentiation detected in each treatment group increased with different extent compared with the control group.Some increases were significant.XD02 had the best inductive effect and the least cytotoxicity.CONCLUSION The results indicates that all four PAE could induce osteogenic differentiation of bMSC.The PAE has the potential to be developed as a dietary supplement or drug for osteoporosis intervention.
关键词
美洲大蠊/提取物/骨质疏松/骨髓间充质干细胞/诱导分化/成骨分化/碱性磷酸酶/钙化Key words
Periplaneta americana/Extract/Osteoporosis/bMSCs/Induced differentiation/Osteogenic differentiation/Alkaline phosphatase/Calcification引用本文复制引用
基金项目
国家自然科学基金资助项目(U21A20409)
出版年
2024
NETL
NSTL
万方数据