摘要
目的 研究多刺绿绒蒿全草中的化学成分.方法 采用正相硅胶柱层析、反相制备色谱分离纯化多刺绿绒蒿80％乙醇提取物中的化学成分,并通过质谱、核磁共振波谱法、薄层色谱确定其结构.结果 从多刺绿绒蒿乙醇提取物中分离得到了15 个化合物,分别鉴定为 15-nonacosanol(Ⅰ)、triacontan-11-ol(Ⅱ)、3β-hydroxy-7α-ethoxy-24β-ethyl-cholest-5-ene(Ⅲ)、β-谷甾醇(Ⅳ)、山奈酚(Ⅴ)、小麦黄素(Ⅵ)、木犀草素(Ⅶ)、芹菜素(Ⅷ)、丁二酰胺(Ⅸ)、金圣草黄素(Ⅹ)、咖啡酸(Ⅺ)、铵羧基丙二酸盐(Ⅻ)、原儿茶酸甲酯(ⅩⅢ)、槲皮素(ⅩⅣ)、3-[(l-carboxyvinyl)oxy]benzoic acid(ⅩⅤ).结论 化合物为Ⅰ、Ⅱ、Ⅺ、Ⅻ、ⅩⅢ、ⅩⅤ为首次从多刺绿绒蒿属植物中分离得到.
Abstract
OBJECTIVE To study the constituents of the plant Meconopsis horridula Hook.f.&Thomson.METHODS Reversed-phase preparative liquid chromatography and normal-phase silica gel column chromatography recrystallization methods were used for the isolation and the purification of compands from the 80％ethanol extracts from M.horridula.Their structures were elucidated by MS and NMR.RESULTS Fifteen compounds were isolated from the ethanol extract of M.horridula.Identified as 15-nonacosanol(Ⅰ),triacontan-11-ol(Ⅱ),3β-hydroxy-7α-ethoxy-24β-ethyl-cholest-5-ene(Ⅲ),β-sitosterol(Ⅳ),kaempferol(Ⅴ),tricin(Ⅵ),luteolin(Ⅶ),apigenin(Ⅷ),succinamide(Ⅸ),chrysoeriol(Ⅹ),caffeic acid(Ⅺ),ammonium carboxymalonate(Ⅻ),methyl 3,4-dihydroxybenzoate(ⅩⅢ),quercetin(ⅩⅣ),and 3-[(l-carboxyvinyl)oxy]benzoic acid(ⅩⅤ).CONCLUSION Compounds Ⅰ,Ⅱ,Ⅺ,Ⅻ,ⅩⅢ and ⅩⅤ have been isolated from the plant for the first time.
基金项目
西藏自治区科技厅科技重大专项(XZ202101ZD0021G)
西藏自治区科技厅科技重大专项(XZ202201ZD0001G01)
西藏自治区科技厅科技重大专项(XZ202201ZD0001G06)
西藏自治区科技厅中央引导地方科技成果转移转化项目(XZ202301YD0014C)
西藏自治区科技厅科技计划项目(XZ202301ZY0009G)
NETL
NSTL
万方数据