篦齿虎耳草的化学成分研究
Study on the chemical constituents of Saxifraga umbellulata var.pectinate
黄娇 1黄静 1刘梦影 1俞雅芮 1张艺 2陈东林1
作者信息
- 1. 四川大学华西药学院,四川成都 610041
- 2. 成都中医药大学民族医药学院,四川成都 611137
- 折叠
摘要
目的 研究篦齿虎耳草Saxifraga umbellulata var.pectinata的化学成分.方法 利用硅胶柱色谱、聚酰胺柱色谱、C18反相柱色谱及Sephadex LH-20凝胶柱色谱分离纯化篦齿虎耳草的干燥全草,采用核磁共振技术并结合理化性质鉴定其化学结构.结果 从篦齿虎耳草95％乙醇提取物中分离得到9个化合物,分别鉴定为:(6R,7E,9R)9-hydroxy-4,7-megastigmadien-3-one-9-O-β-D-glucopyranoside(Ⅰ)、3β,5β,6α-trihydroxy-7-megastigmen-9-one-3-O-β-D-glucopyranoside(Ⅱ)、betulabuside A(Ⅲ)、葫芦素 F(Ⅳ)、雪胆乙素苷(Ⅴ)、没食子酸(Ⅵ)、山奈苷(Ⅶ)、山奈酚3-O-α-L-(3-O-乙酰基)-鼠李吡喃糖基-7-O-α-L-鼠李吡喃糖苷(Ⅷ)、β-胡萝卜苷(Ⅸ).结论 化合物 Ⅰ～Ⅴ为首次从虎耳草属植物中发现.
Abstract
OBJECTIVE To study the chemical constituents from the whole plants of Saxifraga umbellulata var.pectinate.METHODS The constituents were isolated and purified by silica gel,polyamide,C18 reversed phase and Sephadex LH-20 column chromatographies,and their structures were identified by NMR spectra and physical-chemical constants.RESULTS Nine compounds were isolated from the 95％ethanol extract of S.umbellulata var.pectinate and identified as(6R,7E,9R)-9-hydroxy-4,7-megastigmadien-3-one-9-O-β-D-glucopyranoside(Ⅰ),3β,5β,6α-trihydroxy-7-megastigmen-9-one-3-O-β-D-glucopyranoside(Ⅱ),betulabuside A(Ⅲ),cucurbitacin F(Ⅳ),hemsamabilinin B(Ⅴ),gallic acid(Ⅵ),kaempferitrin(Ⅶ),kaempferol 3-O-α-L-(3-O-acetyl)-rhamnopyranosyl-7-O-α-L-rhamnopyranoside(Ⅷ),and β-carotene(Ⅸ).CONCLUSION Compounds Ⅰ-Ⅴ have been isolated from genus Saxifraga for the first time.
关键词
藏药/篦齿虎耳草/降倍半萜苷/单萜苷/四环三萜/黄酮苷/甾体Key words
Tibetan medicine/Saxifraga umbellulata var.pectinata/Nor-sesquiterpene glycosides/Monoterpenoid glycosides/Tetracyclic triterpenoids/Flavonoid glycosides/Steroids引用本文复制引用
基金项目
国家自然科学基金资助项目(81973573)
出版年
2024
NETL
NSTL
万方数据