Preparation of naringenin platelet-mimetic nanoparticles and the protective effect on myocardial ischemia-reperfusion injury
OBJECTIVE To prepare naringenin loaded platelt-mimetic nanoparticles(MSN-Nar@PLTM)and to investigate their physicochemical properties as well as their protective effects against myocardial ischemia-reperfusion injury.METHODS Platelet membranes were extracted by gradient centrifugation with repeated freezing and thawing,and nanoparticles were prepared by impregnation method and sonication.The drug loading,encapsulation rate,particle size,potential,morphology,and biosafety of the nanoparticles were also characterized to verify the integrity of the proteins on the surface of platelet membranes and the therapeutic effect of the nanomaterials on myocardial ischemia-reperfusion injury.RESULTS MSN-Nar@PLTM was 139.57±0.96 nm in diameter.The zeta potential was-21.80±0.15 mV,the loading capacity was 15.3％,and the encapsulation rate was 30.7％.MSN-Nar@PLTM had an intact membrane structure,good biosafety,and demonstrated a protective effect on damaged cardiomyocytes.CONCLUSION MSN-Nar@PLTM platelet-mimetic delivery system,which can load naringenin efficiently,addresses the issues of poor solubility and slow dissolution of naringenin after oral administration,improves its bioavailability,and has protective effects against myocardial ischemia-reperfusion injury.
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