Study on fingerprint and optimization of extraction process of Qulinghuazhuo prescription
OBJECTIVE To establish an HPLC fingerprint of Qulinghuazhuo prescription and to optimize its extraction process.METHODS A Sapphire C18 column(250 mm ×4.6 mm,5 μm)was used with mobile phase of 0.1％phosphoric acid water(A)and acetonitrile(B)for gradient elution at a flow rate of 1.0 mL·min-1 under the column temperature of 45 ℃,and the detection wavelength was 240 nm.Orthogonal experiments were designed to investigate the effects of extraction solvent,extraction time and solid-liquid ratio on the influence of the content of main components.RESULTS The fingerprint of Qulinghuazhuo prescription was established and six important chromatographic peaks were identified.The results of similarity evaluation showed that the quality of ten batches of Qulinghuazhuo prescription had some differences.The optimal extraction process was ultrasonic extracting Qulinghuazhuo prescription with 100％methanol at the ratio of 1 g∶6 mL for 90 min.CONCLUSION The fingerprint of Qulinghuazhuo prescription was successfully established and the extraction process was optimized,which lays the foundation for the research on the material basis of medicinal effect and quality control of the prescription.
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