Determination of nucleosides in mulberry branches by LC-MS/MS
OBJECTIVE To determine and analyze the 10 nucleosides in mulberry branches of different varieties from different origins.METHODS The mulberry branch samples were extracted by ultrasonic extraction in pure water,and then gradient elution was performed on column(150 mm ×4.6 mm,3.5 μm)with methanol-ammonium acetate solution as mobile phase.The samples were then determined by LC-MS/MS technique in positive ion mode.The results were analyzed by variance analysis,correlation analysis,principal component analysis and cluster analysis using SPSS 20.0 statistical software.RESULTS 10 nucleosides were detected in 18 batches of mulberry branches,with significant differences in total contents ranging from 0.4115 mg·g-1 to 2.3384 mg·g-1.The content of guanosine(0.5401 mg·g-1),adenosine(0.3284 mg·g-1)and cytidine(0.2690 mg·g-1)was higher in mulberry branches from different producing areas and species,and the lowest content was hypoxanthine(1.54 μg·g-1).There were 18 groups of extremely significant positive correlation among nucleosides,and 9 groups of significant positive correlation,indicating that nucleosides promote each other in the accumulation process.The results of principal component analysis showed that the comprehensive score of chicken mulberry(S4)in Anlan town,Banan District was the highest,followed by Chicken mulberry(S8)in Huangjueya town,Beibei District(S13)and Chicken mulberry(S14)in Fengjie Xinmin town.The cluster analysis results showed that the chicken mulberry(S4)from Bolan town,Banan District was in the same category,the chicken mulberry(S8)from Huangjueya Town,Nanan District,and the other 16 batches of mulberry branch samples were in the same category.CONCLUSION There are high levels of nucleosides in mulberry branches of different varieties from different origin,and the quality of chicken mulberry was better than that of Huasang and mulberry,which can be developed and utilized.
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