Determination of the content for Hydrochlorothiazide tablets by HPLC with ethanol-water as mobile phase
OBJECTIVE To determine the content of Hydrochlorothiazide tablets by HPLC method with ethanol-water as the elution system.METHODS 0.1 mol·L-1 sodium dihydrogen phosphate(pH3.0)-ethanol(88∶12)was used as mobile phase.Welch Ultimate C18 column was used as the chromatographic column.The column temperature was 40℃;the flow rate was 1.5 mL·min-1and the detection wavelength was 271 nm.RESULTS Hydrochlorothiazide injection volume had a good linear relationship with peak area in the range of 0.0527-1.3185 μg,and the average recovery was 98.87％.CONCIUSION The method is environmentally friendly,accurate and can replace the current HPLC method for the determination of Hydrochlorothiazide tablets.
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