乙醇-水为流动相的HPLC法测定氢氯噻嗪片的含量
Determination of the content for Hydrochlorothiazide tablets by HPLC with ethanol-water as mobile phase
王静霞 1魏长勇 2余敏灵2
作者信息
- 1. 四川工商职业技术学院轻工工程学院,四川都江堰 611830
- 2. 乐山市食品药品检验检测中心四川省药品监督管理局中药质量研究重点实验室,四川乐山 614000
- 折叠
摘要
目的 通过升高柱温降低柱压,建立较大流速的以乙醇-水为洗脱系统的HPLC法来测定氢氯噻嗪片的含量.方法 以0.1 mol·L-1磷酸二氢钠(用磷酸调pH3)-乙醇(88∶12)为流动相,采用Welch Ultimate C18色谱柱(250 mm × 4.6 mm,5 μm),柱温40℃,流速1.5 mL·min-1,检测波长271 nm.结果 0.0527～1.3185μg氢氯噻嗪与峰面积呈良好的线性关系,平均回收率为98.87％.结论 所用方法环保、准确,能替代氢氯噻嗪测定的现行HPLC法.
Abstract
OBJECTIVE To determine the content of Hydrochlorothiazide tablets by HPLC method with ethanol-water as the elution system.METHODS 0.1 mol·L-1 sodium dihydrogen phosphate(pH3.0)-ethanol(88∶12)was used as mobile phase.Welch Ultimate C18 column was used as the chromatographic column.The column temperature was 40℃;the flow rate was 1.5 mL·min-1and the detection wavelength was 271 nm.RESULTS Hydrochlorothiazide injection volume had a good linear relationship with peak area in the range of 0.0527-1.3185 μg,and the average recovery was 98.87％.CONCIUSION The method is environmentally friendly,accurate and can replace the current HPLC method for the determination of Hydrochlorothiazide tablets.
关键词
洗脱系统/乙醇/氢氯噻嗪/高效液相色谱法/含量测定/绿色环保Key words
Elution system/Ethanol/Hydrochlorothiazide/HPLC/Content determination/Environmental protection引用本文复制引用
基金项目
四川药品监督管理局科技计划项目(2021012)
出版年
2024
NETL
NSTL
万方数据