Determination of toddalolactone in Zanthoxylum nitidum Chinese medicinal toothpaste by HPLC
OBJECTIVE To detect toddalolactone in Zanthoxylum nitidum Chinese medicinal toothpaste by HPLC.METHODS Zorbax SB-C18(250 mm x4.6 mm,5 pm)was used for sample analysis.0.1％formic acid aqueous solution-acetonitrile was used as mobile phases for gradient elution,with a flow rate of 1.0 mL·min-1.The temperature of the column was 35 ℃ and the wavelength was 328 nm.RESULTS The standard working curve of toddalolactone had good linear relationship in concentration of 0.5180-8.2880 μg·mL-1,and the correlation coefficient was 0.9997.The average recovery rate was 101.04％.CONCLUSION The method has the advantages of short analytical time,high precision and good repeatability,which can be used to determine the content of nitidine in Zanthoxylum nitidum Chinese medicinal toothpaste.
ToddalolactoneHPLCZanthoxylum nitidum Chinese medicinal toothpasteContent determination
NETL
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万方数据
