Inhibition mechanism of compound MYZ-20 on drug-resistant Pseudomonas aeruginosa
OBJECTIVE To study the inhibitory effect and mechanisms of compound MYZ-20 on drug-resistant Pseudomonas aeruginosa.METHODS The minimum inhibition concentration and the minimum bactericidal concentration of MYZ-20 were determined by the double dilution method.The bactericidal efficacy of MYZ-20 was confirmed by measuring the relative conductivity and extracellular alkaline phosphatase activity,and the inhibitory effect of MYZ-20 on the virulence factors of drug-resistant P.aeruginosa was explored by testing the pyocyanin,green fluorescein,and the formation of biofilm.Additionally,a scalded infection mouse model was established for the determination of the anti-infection and wound healing effects of MYZ-20.RESULTS MYZ-20 killed P.aeruginosaby destroying the membrane structure,significantly reducing the level of bacterial virulence factors,promoting wound repair and reducing the level of inflammation in mice.CONCLUSION MYZ-20 has a strong bactericidal effect on drug-resistant P.aeruginosa,and has anti-inflammatory and wound repair effects.
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