HPLC同时测定沙棘中的6种色胺类生物碱
Simultaneous determination of six tryptamine alkaloids in Hippophae rhamnoides by HPLC
刘振华 1任李成城 1王月 1董琦 2王洪伦 2胡娜2
作者信息
- 1. 青海大学医学院,青海西宁 810001
- 2. 中国科学院西北高原生物研究所中国科学院藏药研究重点实验室青海省藏药研究重点实验室,青海西宁 810008
- 折叠
摘要
目的 采用HPLC法同时测定6种沙棘中的色胺类生物碱.方法 采用Xcharge-C18色谱柱(250 mm ×4.6 mm,5 μm),流动相为甲醇-0.15％三氟乙酸水溶液,梯度洗脱,流速1.0mL·min-1,检测波长280 nm,柱温30 ℃,进样量10 μL.结果 6 种色胺类生物碱的线性范围分别为 6.28～251.40、5.71～228.60、1.14～457.10、0.80～32.00、0.31～12.60、0.46～18.30μg·mL-1(r2＞0.9993);其中,供试品中共检测到4种色胺类生物碱,分别是5-羟色胺、6-hydroxy-l-methyltetrahydro-β-carboline-1-carboxylic acid、shepherdine、L-色氨酸;平均加样回收率分别为 93.63％、89.86％、91.58％、90.91％,RSD均＜3.00％.结论 所用方法简便、可靠、重复性好、结果准确.
Abstract
OBJECTIVE To simultaneous determine the of 6 tryptamine alkaloids in Hippophae rhamnoides L.by HPLC.METHODS The separation was performed on Xcharge-C18(250 mm × 4.6 mm,5 μm)column with mobile phase consisted of methanol-0.15％trifluoroacetic acid solution by gradient elution at the flow rate of 1.0 mL·min-1.The wavelength was set at 280 nm,column temperature was 30 ℃ and the injection volume was 10 μL.RESULTS The linear ranges of 6 tryptamine alkaloids were 6.28-251.40 μg·mL-1,5.71-228.60 μg·mL-1,1.14-457.10 μg·mL-1,0.80-32.00 μg·mL-1,0.31-12.60 μg·mL-1and 0.46-18.30 μg·mL-1(r2＞0.9993),respectively.Four kinds of tryptamine alkaloids were detected in the test samples,which were 5-hydroxytryptamine,6-hydroxy-l-methyltetrahydro-β-carboline-1-carboxylic acid,shepherdine and L-tryptophan.The average recoveries were 93.63％,89.86％,91.58％and 90.91％,respectively.The RSDs were less than 3.00％.CONCLUSION The method is simple and reliable,with good repeatability and accurate results.
关键词
沙棘/高效液相色谱法/色胺类生物碱/5-羟色胺/L-色氨酸/Shepherdine/含量测定Key words
Hippophae rhamnoides L./HPLC/Tryptamine alkaloids/5-HT/L-Tryptophan/Shepherdine/Content determination引用本文复制引用
基金项目
中国科学院王宽诚率先人才计划产研人才扶持项目(2021)()
青海省创新平台建设专项(2021-ZJ-T05)
出版年
2024
NETL
NSTL
万方数据