OBJECTIVE To identify the pharmacognostical characteristics of Anaphalis lactea.METHODS The origin identification,morphological identification,microscopic evaluation,TLC and DNA barcoding analysis were carried out.RESULTS Microscopic characteristics of the transverse section and powders were obvious.TLC spots were clear and well-separated.By PCR amplification and sequencing of ITS sequence,ITS sequence was obtained for the first time.The GenBank registration number was OQ096626,and its DNA barcode was determined.CONCLUSION The study can provide a reference for the identification,resource development and quality control of A.lactea.
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