首页|GC-MS结合化学计量法分析全缘金粟兰的挥发性成分

GC-MS结合化学计量法分析全缘金粟兰的挥发性成分

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目的 分析比较全缘金粟兰根、茎、叶、花中的挥发性成分.方法 采用环己烷回流提取全缘金粟兰根、茎、叶、花中的挥发性成分,并运用峰高归一化法计算和比较各成分的相对含量.结果 共鉴定出73种挥发性成分,包括萜类、醇类、烯烃类、酯类、酮类等.其中,根、茎、叶、花中分别鉴定出37、17、27、34种;4个部位中特有挥发性成分分别为29、3、4、15种.此外,共有成分1-辛烯-3-醇在茎、叶、花中的含量均超过12％,而(E)-β-金合欢烯则低于5％.结论 主成分分析与聚类分析结果表明:全缘金粟兰的叶与花聚为一组,再与茎聚为一组,最终与根聚为一组.
Analysis of volatile components in the whole plant of Chloranthus holostegius by GC-MS combined with chemical stoichiometry
OBJECTIVE To compare and analyze the volatile components of roots,stems,leaves and flowers of Chloranthus holostegius.METHODS The volatile components of the roots,stems,leaves and flowers were extracted by Cyclohexane reflux,and the relative contents of each component were calculated and compared by using the peak height normalization method.RESULTS A total of 73 volatile components were identified,including terpenes,alcohols,alkenes,esters and ketones.Among them,37 volatile components were identified from roots,17 from stems,27 from leaves and 34 from flowers.29 specific volatile components were identified from roots,3 from stems,4 from leaves and 15 from flowers,respectively.In addition,there are two common components:1-octene-3-ol is more than 12％in stems,leaves and flowers,and(E)-β-acacia is less than 5％.CONCLUSION The results of Principal component analysis(PCA)and Cluster analysis(HCA)showed that the leaves and flowers were grouped together,then the stems were grouped together,and finally the roots were grouped together.

Chloranthus holostegiusRootStemsLeavesFlowersVolatile componentsGC-MSPCA analysisHCA analysisChemical stoichiometry

李军、李琴、刘婷婷、何席呈、洪开文、蔡攀峰

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贵州中医药大学药学院贵州省苗医药重点实验室,贵州贵阳 550025

安顺职业技术学院应用医药系,贵州安顺 561099

全缘金粟兰 根 茎 叶 花 挥发性成分 气相色谱-质谱联用 主成分分析 聚类分析 化学计量法

2021年国家自然科学基金后补助资金科研创新探索专项2021年国家自然科学基金后补助资金科研创新探索专项2021年国家自然科学基金后补助资金科研创新探索专项

2018TFL170810508贵中医科院内[2019]75号3040-042200001-75

2024

10.13375/j.cnki.wcjps.2024.04.015

华西药学杂志
四川大学,四川省药学会

华西药学杂志

CSTPCD北大核心
影响因子：0.624
ISSN：1006-0103
年,卷(期)：2024.39(4)
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