摘要
目的 分析比较全缘金粟兰根、茎、叶、花中的挥发性成分.方法 采用环己烷回流提取全缘金粟兰根、茎、叶、花中的挥发性成分,并运用峰高归一化法计算和比较各成分的相对含量.结果 共鉴定出73种挥发性成分,包括萜类、醇类、烯烃类、酯类、酮类等.其中,根、茎、叶、花中分别鉴定出37、17、27、34种;4个部位中特有挥发性成分分别为29、3、4、15种.此外,共有成分1-辛烯-3-醇在茎、叶、花中的含量均超过12%,而(E)-β-金合欢烯则低于5%.结论 主成分分析与聚类分析结果表明:全缘金粟兰的叶与花聚为一组,再与茎聚为一组,最终与根聚为一组.
Abstract
OBJECTIVE To compare and analyze the volatile components of roots,stems,leaves and flowers of Chloranthus holostegius.METHODS The volatile components of the roots,stems,leaves and flowers were extracted by Cyclohexane reflux,and the relative contents of each component were calculated and compared by using the peak height normalization method.RESULTS A total of 73 volatile components were identified,including terpenes,alcohols,alkenes,esters and ketones.Among them,37 volatile components were identified from roots,17 from stems,27 from leaves and 34 from flowers.29 specific volatile components were identified from roots,3 from stems,4 from leaves and 15 from flowers,respectively.In addition,there are two common components:1-octene-3-ol is more than 12%in stems,leaves and flowers,and(E)-β-acacia is less than 5%.CONCLUSION The results of Principal component analysis(PCA)and Cluster analysis(HCA)showed that the leaves and flowers were grouped together,then the stems were grouped together,and finally the roots were grouped together.
基金项目
2021年国家自然科学基金后补助资金科研创新探索专项(2018TFL170810508)
2021年国家自然科学基金后补助资金科研创新探索专项(贵中医科院内[2019]75号)
2021年国家自然科学基金后补助资金科研创新探索专项(3040-042200001-75)