Study on the mass spectrometry decomposition pattern of citrus flavonoids
OBJECTIVE To study the mass spectrometric fragmentation pattern of flavonoids in citrus.METHODS The mass spectral cleavage patterns of flavonoids were summarized by determining the secondary mass spectra of 14 representative flavonoids in citrus,determining the elemental compositions of the ions based on the precise mass-to-charge ratio,and comprehensively resolving the fragmentation ions and cleavage pathways.RESULTS The cleavage of citrus flavonoid glycosides in mass spectra was the sequential removal of two monosaccharides,and the cleavage modes of aglycons include de-CH3,de-CO,and C-ring opening.In the cleavage of the aglycons,there were differences in the appearance of the above various pathways in the structures of dihydroflavonoids,flavonoids and flavonols.CONCLUSION The electrospray mass spectrometric cleavage of flavonoids in citrus has a certain pattern,and these characteristic fragment ions can be used as a basis for identifying flavonoid phenotypes in complex systems.
CitrusFlavonoidsHigh resolution mass spectrometryCollision induced ionizationLC-MSIons for diagnosisFragmentation pattern
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