Determination of four aristolochic acid components in Zaizao pills by UPLC-MS/MS
OBJECTIVE To determine the aristolochic acid Ⅰ,aristolochic acid Ⅱ,aristolochic acid Ⅲ and aristolactam Ⅰin Zaizao pills by UPLC-MS/MS method.METHODS The chromatography was performed on Waters Acquity BEH UPLC C18 column(100 mm × 2.1 mm,1.7 μm)with methanol and 0.1％formic acid aqueous solution(containing 5 mmol·L-1 ammonium formate)at a gradient elution rate of 0.3 mL·min-1.The quantitative analysis was carried out by external standard method in multiple reaction monitoring mode with positive electrospray ionization.RESULTS The 4 aristolochic acids components showed good linearity in the range of test concentration,with correlation coefficients greater than 0.9990.The average recoveries were between 88.4％and 102.5％,with RSD less than 5％.CONCLUSION The established method is simple,sensitive,accurate,which can provide reference for the quality control of Zaizao pills.
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