OBJECTIVE To investigate the therapeutic effects and mechanism of Fangfeng Tongsheng powder(FTP)on mice with polycystic ovarian syndrome(PCOS).METHODS The PCOS mice model was established by feeding a high-fat diet with gavage Letrozole 1.4 mg·kg-1.Blood and ovarian tissue from model mice treated with FTP were collected for the biochemical,pathological and immunological tests.The potential targets of FTP for the treatment of PCOS were analyzed by Network pharmacology.RESULTS FTP significantly reduced the fasting blood glucose level,increased the number of corpus luteum and the thickness of granulosa cell layer in ovarian tissues,up-regulated the levels of estradiol and follicle stimulating hormone,and reduced the level of IL-6 in the serum of PCOS mice.It also up-regulated the expression level of AKT and down-regulated the expression level of VEGFA in ovarian tissues.Network pharmacological analysis showed that AKT,IL-6 and VEGFA are the core targets of FTP in the treatment of PCOS.CONCLUSION FTP can improve the pathological damage of ovarian tissues and hormone disorders in PCOS mice,and its mechanism is related to the regulation of AKT,IL-6 and VEGF expression.
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