OBJECTIVE To optimize the purification process of anti-inflammatory constituents group from Rheum tanguticum(RTAC)and to study its anti-inflammatory effects.METHODS The 10 kinds including sennoside B,ononin,sennoside A,emodin 8-O-glucoside,mangostensin,aloe emodin,rhein,emodin,chrysophanol,and emodin methyl ether were used as RTAC.The HPLC analysis was performed on an Agilent TC-C18 chromatography column(250 mm ×4.6 mm,5 µm).The mobile phase consisted of 0.1％formic acid aqueous solution-methanol.The flow rate was 1 mL·min-1,and the column temperature was 30 ℃ with the injection volume of 10 μL and the detection wavelength of 280 nm.The extraction,separation and purification of RTAC were used by the solvent method and macroporous adsorption resin method,and the activity of RTAC was investigated.RESULTS 10 components in the RTAC of mainly existed in the dichloromethane and water saturated n-butanol parts.The water saturated n-butanol part was further purified with macroporous adsorption resin,and its mass fraction increased from 6.27％to 40.08％.The purified RTAC with each dose significantly reduced the swelling of mice ears,feet,and cotton ball granulation,and the high-dose group had significantly better effects than the pre-purified R.tanguticum methanol extracts group.CONCLUSION This method can be used to separate and purify the RTAC,providing a basis for the further development and utilization of Rheum tanguticum.
关键词
唐古特大黄/抗炎活性成分/含量测定/大孔吸附树脂/分离/纯化工艺/抗炎活性
Key words
Rheum tanguticum/Anti-inflammatory active constituents/Content determination/Macroporous adsorption resin/Separate/Purification process/Anti-inflammatory activity
维普
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