OBJECTIVE To study the protective effects and mechanisms of Balanophora involucrata Hook.f on drug-induced liver injury(DILI)by Network pharmacology and experimental validation.METHODS Through literature searches,the chemical composition of B.involucrata was compiled and the pharmacological mechanism was predicted using the diverse databases.DILI mice model was established by Acetaminophen(APAP),with Bifendate as a positive drug group.The study encompassed macroscopic specimens,histopathological examination,biochemical assays,immunohistochemistry,and molecular biology analyses to unravel the therapeutic effects and pharmacological mechanisms of polysaccharide of B.involucrata(BPS)on DILI.RESULTS The sanctuone,β-eudesmol,and β-sitosterol as the principal active components of B.involucrata in regulating DILI.Core targets included TNF,MAPK3,PTGS2,BCL2L1.GO functional enrichment analysis implicated processes associated with apoptosis regulation.KEGG analysis highlighted the involvement of apoptosis-related signaling pathways.The liver index,ALT,and AST levels of DILI model mice were significantly reduced,and the liver tissue pathology and inflammatory infiltration were improved by BPS treated.Additionally,BPS mitigated hepatocyte apoptosis by inhibiting caspase 3 activation,suppressing Bax expression,and elevating Bcl-2 expression.CONCLUSION B.involucrata could prevent and treat APAP-induced liver injury by regulating cell apoptosis through the Bax/Bcl-2/caspase 3 signaling pathway.
维普
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