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滨蒿总黄酮抗呼吸道病毒的体外活性评价

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目的 考察滨蒿总黄酮体外抗呼吸道合胞病毒、柯萨奇病毒B3株、甲型流感病毒H1N1的作用.方法 采用呼吸道合胞病毒感染Hep2/A549细胞、柯萨奇病毒B3株感染Hep2细胞、HlN1感染A549/THP1细胞模型,细胞病变评分法和Viral ToxGlo法检测滨蒿总黄酮抗呼吸道病毒的作用;ELISA法检测滨蒿总黄酮对病毒感染细胞上清中白介素-1β(IL-1β)、IL-6和肿瘤坏死因子α(TNF-α)的含量.结果 滨蒿总黄酮在体外对呼吸道病毒有明显的抑制作用,呈量效关系;滨蒿总黄酮还通过下调IL-6和TNF-α的表达来参与病毒介导的炎症反应.结论 滨蒿总黄酮在体外表现出良好的抑制呼吸道病毒作用.
Anti-respiratory virus effects of the total flavonoids from Artemisia scoparia in vitro
OBJECTIVE To investigate the effects of total flavonoids from Artemisia scoparia Waldst.et Kit.(ASTF)on respiratory syncytial virus(RSV),Coxsackie virus B3(CBV3)and influenza A(H1N1)in vitro.METHODS Models(RSV-infected Hep2/A549 cells,CBV3-infected Hep2 cells and H1 N1-infected A549/THP1 cells)were used to detect the anti-respiratory virus effect of ASTF in vitro,Cytopathic effect scoring and Viral ToxGlo were used to evaluate the anti-respiratory virus effect of ASTF;ELISA method was further conducted to elucidate the impact of ASTF on the secretion of inflammatory cytokines IL-1 β,IL-6 and TNF-α in the supernatant infected by respiratory virus.RESULTS ASTF showed an inhibitory effect on respiratory viruses in vitro.With increasing concentration,the antiviral effect was enhanced in a dose-dependent way.ASTF was also involved in the virus-mediated inflammatory response by decreasing the expression of IL-6 and TNF-α.CONCLUSION ASTF demonstrated good inhibitory effect on respiratory virus in vitro.

Total flavonoids of Artemisia scopariaA549Hep2THP1Inflammatory cytokinesRespiratory syncytial virusCoxsackie virus B3H1N1 influenza virus

马雪萍、史玉柱、王雪

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新疆维吾尔自治区药物研究所维吾尔药重点实验室,新疆乌鲁木齐 830011

滨蒿总黄酮 人肺癌A549细胞 人喉癌Hep2细胞 人急性单核THP-1细胞 炎性细胞因子 呼吸道合胞病毒 柯萨奇病毒B3株 甲型流感病毒H1N1

自治区创新环境(人才、基地)建设专项-科技创新基地建设(资源共享平台建设)项目

PT2305

2024

10.13375/j.cnki.wcjps.2024.05.008

华西药学杂志
四川大学,四川省药学会

华西药学杂志

CSTPCD北大核心
影响因子：0.624
ISSN：1006-0103
年,卷(期)：2024.39(5)