OBJECTIVE To optimize the extraction process of total flavonoids from Conioselinum tataricum Hoffmann,and to evaluate their in vitro antioxidant and hypoglycemic activities.METHODS Single factor and response surface experiments were used to optimize the extraction process of total flavonoids from C.tataricum.The distribution and content of the main flavonoid aglycones were identified by HPLC.The antioxidant capacity of total flavonoids from C.tataricum was evaluated by DPPH free radical scavenging rate,hydroxyl radical scavenging rate,and ABTS free radical scavenging rate.The in vitro hypoglycemic activity was evaluated by the α-glucosidase inhibition experiment.RESULTS After optimization,the total flavonoids extracted from C.tataricum was 21.36 mg·g-1.Six major flavonoids were identified from the total flavonoid extracts.Among them,the higher contents were puerarin,luteolin,and quercetin,which were 6.972,2.921,and 3.257 mg·g-1,respectively.All of them showed excellent scavenging capabilities and a dose dependence within a certain concentration range.When the total flavonoid mass concentration of C.tataricum was 1.4846 mg·mL-1,the α-glucosidase inhibition rate reached 91.23％.CONCLUSION The established method for extracting total flavonoids from C.tataricum has high accuracy and reliability,and can be used to extract and predict the amount of total flavonoids extracted from C.tataricum.
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