OBJECTIVE To explore the effects and mechanism of nutgall water extracts(NWE)on ulcerative colitis(UC).METHODS The UC rat model was established using 2,4,6-trinitrobenzene sulfonic acid.UC model rats were randomly divided into model group,Mesalazine group,and low,medium,and high dose of NWE groups.A normal control group was previously established.The normal group rats were given water by gavage,and each treatment group was given corresponding concentrations of drugs by gavage once a day for 15 consecutive days.Disease activity index(DAI)scoring was performed regularly during the administration process.After dissection,Luk scoring was performed.Cell classification was performed using a blood cell analyzer.Expression levels of related inflammatory factors in colon tissue were detected by ELISA,and the pathological changes in colon tissue were observed by HE staining.The mRNA transcription levels of transforming growth factor,intestinal trefoil factor 3 and β-defensin 2 in colon tissues were detected by RT-PCR.RESULTS NWE significantly reduced DAI and Luk scores in UC rats,regulated the expression levels of inflammatory and repair factors,and improved the degree of colon lesions in UC rats.CONCLUSION NWE may promote the healing of colon ulcers by inhibiting immune inflammatory response,regulating the expression levels of pro-inflammatory and repair factors,and thus exerting anti-ulcerative colitis effects.
维普
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