OBJECTIVE To establish an UPLC-MS/MS method for the simultaneous determination of 19 plant growth regulator residues in Huai Dioscoreae Rhizoma,Huai Rehmanniae Radix,Huai Achyranthis bidentatae Radix and Huai Chrysanthemi Flos,known as four major Huaiqing Chinese medicines.METHODS A Capcell PAK cation exchange mixed C18 column(15 cm ×2.0 mm,5 µm)was used,with 0.1％formic acid solution(5 mmol·L-1 ammonium formate)as mobile phase A and acetonitrile-0.1％formic acid solution(5 mmol·L-1 ammonium formate)(95∶5)as mobile phase B,and gradient elution was carried out at a flow rate of 0.3 mL·min-1,and column temperature of 40 ℃.RESULTS The 19 plant growth regulator residues showed good linearity,with determination limits of 0.05-19.35 ng·mL-1 and r＞0.9900,respectively.At three different mass fraction levels,the average recovery rates were in the range of 79％-105％,with the RSDs of 0.78％-13.40％(n=6),respectively.CONCLUSION This method is accurate,rapid and sensitive,which can be used for the residue identification and content determination of 19 plant growth regulators in Huai Dioscoreae Rhizoma,Huai Rehmanniae Radix,Huai Achyranthis bidentatae Radix and Huai Chrysanthemi Flos.
关键词
四大怀药/怀山药/怀地黄/怀牛膝/怀菊花/植物生长调节剂/残留/超高效液相色谱-串联质谱法
Key words
Four major Huaiqing Chinese medicine/Huai Dioscoreae Rhizoma/Huai Rehmanniae Radix/Huai Achyranthis bidentatae Radix/Huai Chrysanthemi Flos/Plant growth regulator/Residue/UPLC-MS/MS
维普
万方数据