OBJECTIVE To simultaneously determine nine chemical components in Dracocephalum heterophyllum Benth.,including neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,acteoside,luteolin-7-O-glucoside,rosmarinic acid,diosmetin 7-O-β-D-glucuronide,quercetin and luteolin by UPLC.METHODS The analysis was performed on a Zorbox SB-C18(100 mm ×4.6 mm,1.8 µm)column with the mobile phase composed of 0.1％formic acid methanol-0.1％formic acid water solution by gradient elution at a flow rate of 0.3 mL·min-1.The wavelength was set at 340 nm with the column temperature of 35 ℃ and the injection volume of 2 µL.RESULTS All of the 9 components have been well separated and exhibited good linear relationships(r2＞0.9978).The average recoveries of nine compounds were in the range of 90.25％-112.61％,and the RSD values were less than 1.25％.CONCLUSION This method is simple and reliable,with good repeatability and accurate results,which could provide a reference for the quality control of D.heterophyllum.
维普
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