首页|UPLC法测定不同产地异叶青兰中的9种成分

UPLC法测定不同产地异叶青兰中的9种成分

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目的 采用超高效液相色谱法同时测定异叶青兰中新绿原酸、绿原酸、隐绿原酸、毛蕊花糖苷、木犀草苷、迷迭香酸、香叶木素-7-O-葡萄糖醛酸苷、槲皮素、木犀草素等化学成分的含量.方法 采用Zorbox SB-C18色谱柱(100 mm × 4.6 mm,1.8 μm),流动相为0.1％甲酸甲醇溶液-0.1％甲酸水溶液,梯度洗脱,流速0.3 mL·min-1,柱温35℃,检测波长340 nm,进样量2 µL.结果 9种成分分离较好,线性关系均良好(r2＞0.9978),平均加样回收率90.25％～112.61％,RSD均＜1.25％.结论 所用方法简便、重复性好、结果准确,可为异叶青兰的质量控制提供参考.
Determination of nine chemical components in Dracocephalum heterophyllum from different regions by UPLC
OBJECTIVE To simultaneously determine nine chemical components in Dracocephalum heterophyllum Benth.,including neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,acteoside,luteolin-7-O-glucoside,rosmarinic acid,diosmetin 7-O-β-D-glucuronide,quercetin and luteolin by UPLC.METHODS The analysis was performed on a Zorbox SB-C18(100 mm ×4.6 mm,1.8 µm)column with the mobile phase composed of 0.1％formic acid methanol-0.1％formic acid water solution by gradient elution at a flow rate of 0.3 mL·min-1.The wavelength was set at 340 nm with the column temperature of 35 ℃ and the injection volume of 2 µL.RESULTS All of the 9 components have been well separated and exhibited good linear relationships(r2＞0.9978).The average recoveries of nine compounds were in the range of 90.25％-112.61％,and the RSD values were less than 1.25％.CONCLUSION This method is simple and reliable,with good repeatability and accurate results,which could provide a reference for the quality control of D.heterophyllum.

Dracocephalum heterophyllum Benth.UPLCContent determinationChlorogenic acidQuercetinActeosideLuteolin-7-O-glucosideRosmarinic acidLuteolinDiosmetin 7-O-β-D-glucuronide

曹璐、胡娜、石秘、宋玉、王洪伦

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中国科学院西北高原生物研究所中国科学院藏药研究重点实验室青海省藏药研究重点实验室,青海西宁 810008

中国科学院大学,北京 100049

异叶青兰 超高效液相色谱法 含量测定 绿原酸 槲皮素 毛蕊花糖苷 木犀草苷 迷迭香酸 木犀草素 香叶木素-7-O-葡萄糖醛酸苷

青海省创新平台建设专项

2021-ZJ-T05

2024

10.13375/j.cnki.wcjps.2024.05.015

华西药学杂志
四川大学,四川省药学会

华西药学杂志

CSTPCD北大核心
影响因子：0.624
ISSN：1006-0103
年,卷(期)：2024.39(5)