Effects of Qingfei Paidu decoction on the activity and mRNA expression of drug metabolizing enzymes in rat liver
OBJECTIVE To study the effects of Qingfei Paidu decoction(QPD)on the activity and mRNA expression of major drug-metabolizing enzymes in rat liver.METHODS After 7 days of continuous intragastric administration of QPD,rats were injected with probe drugs,and the concentrations of probe drugs in plasma were determined by HPLC.The influence of QPD on the activities of cytochrome P450(CYP450)was investigated by comparing plasma pharmacokinetics of probe drugs between control and QPD groups.The effects of QPD on the rat liver UGTs activity were studied through ELISA.The relative expression levels of CYP450 and UGTs mRNA were detected by real-time quantitative PCR(RT-qPCR).RESULTS QPD could significantly induce the expression and activity of CYP2C9,CYP2E1,and CYP2C19,while inhibit the enzyme activity,and mRNA expression of CYP1A2.The result also showed that the activity of UGT1A2,UGT1 A3,and UGT1A8 was increased and the activity of UGT2B7,and UGT1 A9 was inhibited.CONCLUSION QPD has selective regulations on major drug-metabolizing enzymes in rat liver.
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