Determination and pharmacokinetic study of Mitotane in rats plasma
OBJECTIVE To determinate the Mitotane in rats plasma and to study perform pharmacokinetics of Mitotane in rats.METHODS The rats plasma samples underwent pretreatment using the organic solvent precipitation method with acetonitrile as the precipitation agent.The concentration of Mitotane was determined using HPLC,and the resulting pharmacokinetic data were analyzed using DAS 3.0 software.RESULTS The regression equation of Mitotane was Y=1.296× 105 X+5.823×103(r2=0.9999),with the linear range of 0.025-8.008 µg·mL-1.The absolute and method recoveries were between 97.64％and 99.98％,and between 99.93％and 102.09％,respectively.The precision RSD was ≤1.01％(n=3).The Cmax of Mitotane iv differed significantly from that of ig.CONCLUSION The HPLC analysis of Mitotane plasma concentration meets the basic requirements for biological samples,and is suitable for the determination of mitotane in rats.Intravenous administration can significantly increase the peak concentration of Mitotane in the organ.
MitotaneHPLCPharmacokineticsIn vivo determinationOrganic solvent precipitationAdrenocortical carcinomaInsecticideBiological sample preparation
万方数据
维普
