OBJECTIVE To establish a quality control method of Veratrum taliense with the active indicators of jervine,veratramine and cyclopamine,and further,to determine the content of representative steroidal alkaloids in V.taliense and study its dynamic accumulations,building a foundation for the research on regulation of steroidal alkaloid biosynthesis and metabolism in V.taliense.METHODS The determination was performed on Ecosil ODS-Extend 100A(250 mm ×4.6 mm,5 µm)column with a mobile phase consisting of acetonitrile(A)-0.03％triethylamine(B)to establish an HPLC method(the sample size was 10 μL)to determine the content of representative steroidal alkaloids in V.taliense.The similarity evaluation system of chromatographic fingerprints of TCM(2012)was used for fingerprint analysis,and SPSS.25 was used for cluster analysis.RESULTS The precision,repeatability,and stability RSD values of HPLC method were all less than 3％,with an average recovery rate of over 98.91％and RSD values of less than 3％.These three steroid alkaloids had a good linear relationship within the range of 125.0-997.6 mg·L-1,with correlation coefficient r2 was＞0.9998.The dynamic accumulation of steroidal alkaloids in V.taliense was regular.The diversity and content of steroidal alkaloids in the underground part were significantly higher than those in the aerial plant,and the content of steroidal alkaloids in the Wulongba population was significantly higher than that in the Liangwangshan population.CONCLUSION The method is efficient and convenient,and can be used for the quantitative detection of jervine,veratramine and cyclopamine in Veratrum taliense.The steroidal alkaloids in V.taliense have different accumulation and transformation rules in different growth periods.Studying their dynamic accumulation establishes a basis for the regulation of steroidal alkaloid biosynthesis and metabolism in V.taliense,and also has guiding value for the artificial cultivation and standardized planting of V.taliense.
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