OBJECTIVE To rapidly identify the chemical constituents in Lepidium apetalum Willd.by UPLC-Q-Exactive Orbitrap MS.METHODS Waters UPLC HSS T3 column(100 mm × 2.1 mm,1.8 μm)was used,with column temperature of 40 ℃.Gradient elution was performed with methanol-0.1％formic acid as the mobile phase,and the high-resolution mass spectrometry data were collected in positive and negative ion modes.RESULTS Based on the retention time,high-resolution accurate molecular weight,MS/MS fragmentation and other information,combined with reference substance data,95 compounds were identified from L.apetalum,including 24 flavonoids,19 fatty acids,14 phenylpropanoids,11 glucosinolates and isothiocyanates,5 uridines,and 22 others.CONCLUSION The method can quickly and relatively resolved the chemical constituents in L.apetalum,summarize the cleavage law of the compounds.This study provides reference and scientific basis for the component analysis,quality control and clinical application of L.apetalum.
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