OBJECTIVE To rapid determine the bongkrekic acid content in 5 Chinese herbs including Liushenqu,Jianqu,Ganoderma,Polyporus,and Sargassum by UPLC-MS.METHODS The samples were soaked in water and extracted with 1％glacial acetic acid acetonitrile solution with oscillating vortex,purified by dispersed solid phase extraction,nitrogen blow-dried and redissolved with acetonitrile solution.The primary and secondary high-precision full scanning was carried out simultaneously by electrospray negative ion of UPLC-MS at the same time,and the exact mass numbers of parent ions and fragment ions were obtained.A mass spectrum database was constructed to achieve rapid qualitative analysis;quantitative analysis was performed by external standard method;and microbial pathogen identification was used for verification.RESULTS The matrix standard liquid of 5 Chinese herbs had a good linear relationship in the concentration range of 30-200 μg·L-1,with correlation coefficient r≥ 0.9917,the limit of detection of 10-20 μg·kg-1,and the limit of quantification of 30-50 µg·kg-1.The average recoveries were 72.9％-118.0％,and RSD was 1.70％-7.40％(n=6).No bongkrekic acid was detected in the samples.Bacteria such as Klebsiella pneumoniae,Enterobacter cloacae complex and Burkholderia cepacia were identified with similar colony characteristics to Burkholderia gladiolus.CONCLUSION This method demonstrates low matrix interference and fast sample analysis.Compared with the traditional method,the experimental results are basically the same,and the test time is greatly shortened.
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