Determination of 10 components in Bushen Yinao capsules by UPLC with multi-wavelength
OBJECTIVE To determine the 10 components in Bushen Yinao capsules(BYC)by UPLC.METHODS The chromatographic column was Acquity UPLC BEH-C18(100 mm × 2.1 mm,1.7 μm).The mobile phase was acetonitrile-0.1％phosphoric acid aqueous solution.Gradient elution was performed at the flow rate of 0.3 mL·min-1,the column temperature was 30 ℃,and the sample volume was 1 μL.The detection wavelengths were 203 nm,250 nm,and 320 nm,respectively.RESULTS The mass concentrations of ferulic acid,spinosin,psoralatin,isopsoralatin,ginsenoside Rg1,ginsenoside Re,harbacoside,ginsenoside Rb1,schisandrin and schisantherin A were 13.15-210.40,2.58-41.35,1.16-18.58,1.64-26.22,6.03-96.41,7.21-115.30,1.28-20.48,24.50-392.00,1.16-18.64,1.16-18.64,2.42-38.68 μg·mL-1.There was a good linear relationship with the peak area,and the r were greater than 0.9998.The average recoveries were 92.8％,92.0％,97.3％,97.8％,91.0％,102.3％,95.3％,99.3％,101.4％,96.7％,and the RSD values were 1.30％,1.78％,2.65％,1.95％,1.86％,3.12％,1.09％,1.35％,1.60％,1.85％,respectively.CONCLUSION The method is simple,reliable and reproducible,and can be used for the determination of 10 components in BYC.
万方数据
维普
