Antibacterial Activity of Dandelion Aqueous Extract Against Vibrio Parahaemolyticus
The aim of this study was to investigate the antibacterial activity and mechanism of action of natural antimicrobial agent dandelion water extract against Vibrio parahaemolyticus.The antibacterial activity was evaluated by determining the minimal inhibitory concentration(MIC),growth curve,and planktonic cell inactivation rate.The antibacterial mechanism was explored by assessing extracellular al-kaline phosphatase and protein leakage,bacterial motility,and inhibition rate of biofilm formation.The results showed that the MIC of Dandelion water extract against V.parahaemolyticus was 26 mg/mL,sig-nificantly inhibiting its growth and proliferation.Under physiological saline conditions,treatment with 1 MIC of dandelion water extract for 3 hours resulted in a 99.04％inactivation rate,with extracellular al-kaline phosphatase activity and protein content increasing by 4.34-fold and 5.61-fold,respectively.Scan-ning electron microscopy revealed changes in bacterial cell morphology induced by Dandelion water ex-tract,effectively inhibiting V.parahaemolyticus swimming and swarming ability.Furthermore,dandelion water extract at 1/4 MIC and 1/2 MIC inhibited biofilm formation of V.parahaemolyticus by 48.59％and 71.31％,respectively.Therefore,Dandelion water extract holds potential for application in control-ling V.parahaemolyticus in aquaculture feed or water.
万方数据
维普
