An analysis of genetic diversity of wild and bred Cyprinus carpio in Tianjin area
Genetic diversity and variation of two wild Cyprinus carpio populations(Zhouhe carp and Chaobaihe carp)and one selected population(C.carpio var.FFRC 2# were assessed)using 12 micro-satellite markers.The number of alleles ranged from 2 to 21 among three populations.The average ef-fective allele number varied between 2.969 6 and 8.916 0.The average expected heterozygosity ranged from 0.636 7 to 0.882 5.The average observed heterozygosity ranged from 0.675 9 to 0.790 6.The average polymorphic information content(PIC)varied between 0.573 2 and 0.872 7 with the highest PIC＞0.5).The pairwise genetic differentiation index was 0.158 8 and 0.210 4 in the wild and the se-lected populations,respectively,indicating that the three populations genetically differentiated signifi-cantly.Analysis of molecular variance(AMOVA)detected a high genetic variation among individuals(86.38％)while variation among populations explained 13.62％of the total.The UPGMA phylogenet-ic tree based on Nei's distance demonstrated that the Zhouhe carp and the Chaobaihe carp are closely related,and two populations of the wild carp had far relationship to the selected population.Overall,genetic variation among wild populations and selected population was significant.The selected popula-tion had a pure genetic germplasm while the wild populations had a high genetic diversity and potential for further selective breeding.
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